세포주 정보

RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%

NCTC 135 medium, 100%

RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%

this grows in chemically defined medium; the cells attach very lightly to the substrate, and as the culture becomes heavy some of the cells will detach into the medium; tested and found negative for ectromelia virus (mousepox)