SK-LU-1
| KCLB NO |
30057
|
|---|---|
| Name |
SK-LU-1
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lung
|
|---|---|
| Species |
human female, 60 years old, Caucasian
|
| Tumorigenecity |
Yes, in immunotolerant rats
|
| Isoenzyme |
Me-2,1;PGM3,1;PGM1,2;ES-D,1;AK-1,1;GLO-1,2;G6PD,B
|
| Karyology |
The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma
|
| D3S1358 |
18
|
|---|---|
| vWA |
16,17
|
| FGA |
21,22
|
| Amelogenin |
X
|
| TH01 |
7
|
| TPOX |
8,10
|
| CSF1P0 |
10
|
| D5S818 |
11
|
| D13S317 |
10
|
| D7S820 |
9
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium (Eagle) in Earle's BSS with nonessential amino acids and 1mM sodium pyruvate, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| subculturing |
Remove medium, add with fresh 0.25% trypsin solution for 1 min, remove trypsin and let the culture sit at room temperature for 5 to 10 mins. Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days
|
| splitratio |
1:2
|
| media change |
twice per week
|
|
Fogh J. et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst.58: 209-214, 1977. Pollack MS et al. HLA-A,B,C and DR alloantigen expression on forty-six cultured human tumor cell lines.J.Natl.Cancer Inst. 66: 1003-1012, 1981
|
|---|
