세포주 정보

LC-540
LC-540
KCLB NO
10043
Name
LC-540
세포주 분양조건
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Origin
testis
Species
rat male, adult
Strain
Fischer
VirusSusceptibility
vesicular stomatitis (Indiana); vaccinia; herpes simplex
Virus Resistance
poliovirus 1
Reverse Transcriptase
positive
Tumorigenecity
yes, in rats
Cellular morphology
epithelial
Production
steroid hormone; estrogen and androgen
Growth Pattern
monolayer
Histopathology
Leydig cell tumor
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
MEM in Earle's BSS with non-essential amino acids and sodium pyruvate (1mM), 90%; heat inactivated fetal bovine serum (FBS) 10%
kclb media
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
note
at passage 52, steroid production was no longer detectable as measured by secretion over a 6 hour period
J Nat'l Cancer Inst, 41:1141-1153, 1968