NCTC 3749
| KCLB NO |
10046.1
|
|---|---|
| Name |
NCTC 3749
|
세포주 분양조건
서비스
수량
-
+
| Origin |
monocyte; macrophage
|
|---|---|
| Species |
mouse Mus musculus
|
| Strain |
DBA/2
|
| VirusSusceptibility |
vesicular stomatitis (Indiana); polyomavirus
|
| Virus Resistance |
poliovirus 1
|
| Reverse Transcriptase |
positive
|
| Cellular morphology |
epithelial
|
| Histocompatibility |
H-2d
|
| Production |
interleukin-1
|
| Growth Pattern |
monolayer
|
| Histopathology |
lymphoma
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
NCTC 135 medium, 100%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Subcultures are prepared by scraping.
|
| note |
This line is a derivate of P388D1; the cells grow in chemically defined medium(RPMI 1640 medium without serum) and produce IL-1 in response to stimulation with phorbol myristic acid(PMA) and lipopolysaccharide(LPS); tested and found negative for ectromelia virus (mousepox)
|
|
Exp Cell Res, 32:212, 1963; J Immunol, 120: 1497, 1978
|
|---|
