SNU-538BL(SNU-538)
| KCLB NO |
00538EBV
|
|---|---|
| Name |
SNU-538BL(SNU-538)
|
세포주 분양조건
서비스
수량
-
+
| Origin |
B cell
|
|---|---|
| Species |
human, asian male, 35 years old
|
| Cellular morphology |
lymphoblast-like
|
| Growth Pattern |
suspension
|
| Depositor |
Jae-Gahb Park, M.D.
|
| D3S1358 |
15,16
|
|---|---|
| vWA |
14,18
|
| FGA |
21,24
|
| Amelogenin |
X,Y
|
| TH01 |
9
|
| TPOX |
9,11
|
| CSF1P0 |
10
|
| D5S818 |
11,12
|
| D13S317 |
8,9
|
| D7S820 |
10
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
|
| splitratio |
1:4
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Lee et al., B-lymphoblastoid cell lines from cancer patients, In Vitro Cell Dev Biol, 1998, 34:97-100
|
|---|
