세포주 정보

HCC-95
HCC-95
KCLB NO
70095
Name
HCC-95
세포주 분양조건
서비스
수량
- +
Origin
lung
Species
human male, 65 years old
Cellular morphology
epithelial
Growth Pattern
monolayer
Histopathology
squamous cell carcinoma
Depositor
Gazdar AF
D3S1358
17
vWA
14,17
FGA
22
Amelogenin
X
TH01
8,9
TPOX
8,11
CSF1P0
12
D5S818
10,11
D13S317
11
D7S820
10
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
media change
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Wistuba II et al., Comparison of features of human lung cancer cell lines and their corresponding tumors, Clin Cancer Res, 1999, 5:991-1000