세포주 정보

SNU-99BL (SNU-99)
SNU-99BL (SNU-99)
KCLB NO
00099B
Name
SNU-99BL (SNU-99)
세포주 분양조건
서비스
수량
- +
Origin
B cell
Species
human, asian male, 53 years old
Cellular morphology
lymphoblast-like
Growth Pattern
suspension
Histopathology
B lymphoblastoid cell line
Depositor
Jae-Gahb Park, M.D.
EstablishedBy
Jae-Gahb Park, M.D.
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing
Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
Lee et al., B-lymphoblastoid cell lines from cancer patients, In Vitro Cell Dev Biol, 1998, 34:97-100