SNU-4254
| KCLB NO |
04254
|
|---|---|
| Name |
SNU-4254
|
세포주 분양조건
서비스
수량
-
+
| Origin |
brain
|
|---|---|
| Species |
human, asian male, 61 years old
|
| Growth Pattern |
monolayer
|
| Option |
culture only
|
| D3S1358 |
15,17
|
|---|---|
| vWA |
16,18
|
| FGA |
22,22.2
|
| Amelogenin |
X
|
| TH01 |
7,10
|
| TPOX |
8,9
|
| CSF1P0 |
9,12
|
| D5S818 |
9,12
|
| D13S317 |
9,10
|
| D7S820 |
8,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask.
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Genome Med. 2022 Aug 11;14(1):88.
|
|---|
