5637
| KCLB NO |
30009
|
|---|---|
| Name |
5637
|
세포주 분양조건
서비스
수량
-
+
| Origin |
urinary bladder
|
|---|---|
| Species |
human male, 63 years old, Caucasian
|
| Tumorigenecity |
Yes, in nude mice
|
| Isoenzyme |
Me-2,1;PGM3,2;PGM1,1;ES D,1;AK1,1;GLO-1,1-2;G6PD,B.
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma
|
| Modal Choromosome No |
67 (hypotriploid)
|
| D3S1358 |
15,17
|
|---|---|
| vWA |
18
|
| FGA |
22
|
| Amelogenin |
X,Y
|
| TH01 |
7,9
|
| TPOX |
8,9
|
| CSF1P0 |
11
|
| D5S818 |
11,12
|
| D13S317 |
11
|
| D7S820 |
10,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, rinse with calcium and magnesium free PBS, add fresh 0.25% trypsin solution for 3 to 5 minutes at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
|
| splitratio |
1:4 to 1:8
|
| media change |
2 to 3 times weekly
|
|
Goodfellow M et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cancer Inst. 59: 221-226, 1977Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. natl.Cancer Inst.Monogr.: 5-9, 1978
|
|---|
