SNU-620
| KCLB NO |
00620
|
|---|---|
| Name |
SNU-620
|
세포주 분양조건
서비스
수량
-
+
| Origin |
stomach; ascites
|
|---|---|
| Species |
human, asian female, 59 years old
|
| Cellular morphology |
scattered floating
|
| Growth Pattern |
suspension
|
| Histopathology |
adenocarcinoma, metastatic
|
| Differentiation |
poorly differentiated
|
| Depositor |
Jae-Gahb Park, M.D.
|
| D3S1358 |
17,18
|
|---|---|
| vWA |
16,18
|
| FGA |
24
|
| Amelogenin |
X
|
| TH01 |
6,7
|
| TPOX |
8,11
|
| CSF1P0 |
10,11
|
| D5S818 |
11
|
| D13S317 |
12
|
| D7S820 |
13
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
|
| note |
CEA, 252.9 ng/10^6 cells in cell lysate, 377 ng/10^6 cells in supernatant; CA19-9, 11.6 unit/10^6 cells in cell lysate, 2.7 unit/10^6 cells in supernatant; TPA, 80275 unit/10^6 cells in cell lysate, 6095 unit/10^6 cells in supernatant.
|
| splitratio |
1:4
|
| media change |
Collect the cells and centrifuge, remove two thirds of medium, add fresh medium (depending on cell density) every 2 to 3 days
|
|
Park et al., Establishment and characterization of human gastric carcinoma cell lines, Int J Cancer. 1997 Feb 7; 70(4):443-449
|
|
|---|---|
|
