SNU-902
| KCLB NO |
00902
|
|---|---|
| Name |
SNU-902
|
세포주 분양조건
서비스
수량
-
+
| Origin |
cervix, uterine
|
|---|---|
| Species |
human, asian female, 57 years old
|
| Abnormal genes |
HPV16, E7 gene, codon 647, AAT->AGT (Asn to Ser)
|
| Virus |
HPV type 16
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma, squamous cell; stage IIB (FIGO)
|
| Depositor |
Jae-Gahb Park, M.D.
|
| Cell viability |
91%
|
| D3S1358 |
16
|
|---|---|
| vWA |
16,17
|
| FGA |
22
|
| Amelogenin |
X
|
| TH01 |
9
|
| TPOX |
8,9
|
| CSF1P0 |
11
|
| D5S818 |
11
|
| D13S317 |
10,12
|
| D7S820 |
10,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
| note |
HPV16, E1 gene, intact, E2 gene, deleted; HPV 16 was presumably integrated into chromosome; HPV16 was not detectable by Southern blot analysis; E6/E7 gene of HPV16 was expressed by Northern analysis.
|
| populaton doubling time |
52 hours
|
| splitratio |
1:4
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Ku et al., Establishment and Characterization of 12 uterine cervical-carcinoma cell lines: Common sequence variation in the E7 gene of HPV-16-positive cell lines. Int J Cancer. 1997 Jul 17; 72(2):313-320
|
|
|---|---|
|
