FB2
| KCLB NO |
21891
|
|---|---|
| Name |
FB2
|
세포주 분양조건
서비스
수량
-
+
| Origin |
B cell; B lymphocyte
|
|---|---|
| Species |
mouse
|
| Strain |
RBF/DnJ (B cell); BALB/c (myeloma)
|
| Isoenzyme |
IgG3; kappa light chain
|
| Cellular morphology |
lymphoblast
|
| Production |
immunoglobulin; monoclonal antibody; against phosphotyrosine
|
| Growth Pattern |
suspension
|
| Histopathology |
hybridoma; mouse(myeloma)
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with 10mM HEPES, 0.039mg/ml thymidine, 176 ng/ml aminopterin, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| note |
spleen cells were fused with FOX-NY myeloma cells; the antibody reacts with several phosphorylated proteins on Western blots and in immunoprecipitation reactions; thymidine and aminopterin are added to the culture medium to stabilize the presence of the immunoglobulin heavy chain locus
|
|
J Biol Chem, 259:7909-7915, 1984
|
|---|
