세포주 정보

SW480; SW-480
SW480; SW-480
KCLB NO
10228.1G
Name
SW480; SW-480
세포주 분양조건
  • 무항생제 배양
서비스
수량
- +
Origin
Species
human
Reverse Transcriptase
negative
Tumorigenecity
yes, in nude mice
Isoenzyme
G6PD,B;PGM1,2;PGM3,1;6PGD,A;PEP-D,1;ES-D,1
Cellular morphology
epithelial
Histocompatibility
HLA A2,B8,B17; blood type A; Rh+
Production
carcinoembryonic antigen (CEA) 0.7ng/10exp6 cells/10 days; keratin; transforming growth factor beta
Growth Pattern
monolayer
Histopathology
adenocarcinoma
Requirement
epidermal growth factor (EGF)
Option
No Antibiotics
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
Leibovitz
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
note
CSAp negative(CSAp-); colon antigen 3, negative; the cells are positive for keratin by immunoperoxidase staining; the cells contain the ras(K-ras) oncogene
Cancer Res. 36:4562-4569, 1976; J. Nat