Daudi
| KCLB NO |
10213.1G
|
|---|---|
| Name |
Daudi
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
lymphoblast
|
|---|---|
| Species |
human male, 16 years old, Black
|
| Receptor |
complement; Fc of Ig G
|
| Reverse Transcriptase |
negative
|
| Tumorigenecity |
yes, , in nude mice; form colonies in agarose
|
| Isoenzyme |
G6PD, B
|
| Karyology |
normal male; diploid; stable
|
| Cellular morphology |
lymphoblast
|
| Growth Pattern |
suspension
|
| Histopathology |
lymphoma
|
| Option |
No Antibiotics
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with 2mM L-glutamine adjusted to contain 1.5g/L sodium bicarbonate, 4.5g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80 %; heat inactivated fetal bovine serum (FBS), 20 %
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Maintain cultures at 6 X 10 exp5 viable cells/ml.
|
|
Gao Y et al . Introduction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the uurktts lymphoma line Daudi. J. Virol. 71: 84-94, 1997. Cuthbert JA and Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997
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