P3/NSI/1-Ag4-1; NS-1
| KCLB NO |
40018
|
|---|---|
| Name |
P3/NSI/1-Ag4-1; NS-1
|
세포주 분양조건
서비스
수량
-
+
| Origin |
B cell; B lymphocyte
|
|---|---|
| Species |
mouse
|
| Strain |
BALB/c
|
| Tumorigenecity |
no
|
| Isoenzyme |
kappa light chain
|
| Cellular morphology |
lymphoblast
|
| Histocompatibility |
H-2d
|
| Production |
immunoglobulin(not secreted)
|
| Growth Pattern |
suspension
|
| Histopathology |
myeloma; plasmacytoma
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| note |
this is a non-secreting clone of P3X63Ag8 (KCLB 40009); kappa chains are synthesized but not secreted; the cells are resistant to 0.1mM 8-azaguanine and do not grow in HAT medium; the cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity; tested and found negative for ectromelia virus (mouse pox)
|
|
Eur J Immunol, 6:511-519, 1976; Cell, 14:9-20, 1978; In Vitro Cell Develop Biol, 24;1223-1228, 1988
|
|---|
