SK-N-MC
| KCLB NO |
30010
|
|---|---|
| Name |
SK-N-MC
|
세포주 분양조건
서비스
수량
-
+
| Origin |
brain
|
|---|---|
| Species |
human female, 14 years old, Caucasian
|
| Tumorigenecity |
Yes, in nude mice and also in hamster cheek
|
| Isoenzyme |
Me-2,2; PGM3,1-2;PGM1,1;ES D,2;AK1,1;GLO-1,1-2;G6PD,B.
|
| Karyology |
Hypodiploidy to pseudodiploidy
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
Neuroblastoma, metastasis to supra-orbital area
|
| D3S1358 |
15
|
|---|---|
| vWA |
17,18
|
| FGA |
21,25
|
| Amelogenin |
X
|
| TH01 |
9.3
|
| TPOX |
9,11
|
| CSF1P0 |
10
|
| D5S818 |
11
|
| D13S317 |
11
|
| D7S820 |
8
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium Eagle with 2mM L-glutamine, 90% and heat inactivated fetal bovine serum (FBS), 10%; antibiotic-free
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| subculturing |
Remove medium, rinse with fresh 0.25 % trypsin solution, remove trypsin and let the culture sit at room temperature(or at 37''C) until the cells detach(about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
|
|
Spengler BA. et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells established in vitro.In Vitro 8: 410, 1973Goodfellow M et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cancer Inst. 59: 221-226, 1977
|
|---|
