RBL-2H3
| KCLB NO |
22256
|
|---|---|
| Name |
RBL-2H3
|
세포주 분양조건
서비스
- 서비스 준비중입니다.
수량
-
+
| Origin |
blood; basophil
|
|---|---|
| Species |
rat
|
| Strain |
Wistar
|
| Receptor |
FcERI (Fc of IgE)
|
| Cellular morphology |
fibroblast
|
| Production |
histamine
|
| Growth Pattern |
monolayer
|
| Histopathology |
leukemia, chemically induced
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Minimum essential medium Eagle with 2mM L-glutamine and Earle's BSS, 85%; heat inactivated fetal bovine serum (FBS), 15%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| subculturing |
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
|
| splitratio |
1:4 to 1:8
|
| media change |
every 2 to 3 days
|
|
Kulczycki AJ et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J.Exp.Med. 139: 600-616, 1974. Barsumian EL et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur.J.Immunol.11: 317-323, 1981Nat.New Biol. 244: 73-76, 1973
|
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