JEG3
| KCLB NO |
30036
|
|---|---|
| Name |
JEG3
|
세포주 분양조건
서비스
수량
-
+
| Origin |
placenta
|
|---|---|
| Species |
human
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
choriocarcinoma
|
| D3S1358 |
15,16
|
|---|---|
| vWA |
16
|
| FGA |
23,24
|
| Amelogenin |
X,Y
|
| TH01 |
9,9.3
|
| TPOX |
8
|
| CSF1P0 |
11,12
|
| D5S818 |
10,11
|
| D13S317 |
9,11
|
| D7S820 |
10,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Eagle's Minimum Essential Medium, 90%; heat inactivated fetal bovine serum (FBS), 10% or Dulbecco's modified Eagle's Medium, 90%, heat inactivated fetal bovine serum (FBS), 10%, antibiotic free
|
| kclb media |
DMEM90%, heat inactivated fetal bovine serum (FBS), 10% or Dulbecco's modified Eagle's Medium, 90%, heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove culture medium and rinse the cell sheet with fresh 0.25 % trypsin. Remove trypsin, and allow the culture to sit at 37''C for 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
|
| splitratio |
1:2
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
