MKN-74
| KCLB NO |
80104
|
|---|---|
| Name |
MKN-74
|
세포주 분양조건
서비스
수량
-
+
| Origin |
stomach
|
|---|---|
| Species |
human male, 37 years old, Mongoloid
|
| Cellular morphology |
polygonal
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma, tubular
|
| Differentiation |
moderately differentiated
|
| Growth rate |
fast
|
| Depositor |
Japanese Collection of Research Bioresources (JCRB)
|
| DateOfUpdated |
1999-02-02
|
| D3S1358 |
16
|
|---|---|
| vWA |
16,20
|
| FGA |
23
|
| Amelogenin |
X
|
| TH01 |
6
|
| TPOX |
8,11
|
| CSF1P0 |
12
|
| D5S818 |
11
|
| D13S317 |
11
|
| D7S820 |
9
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Cells are washed once with PBS(-) and treated with 0.02% EDTA and 0.25% trypsin for 10-15 min at 37
|
| note |
Mycoplasma-eliminated cells by MC-210
|
| populaton doubling time |
42-46 hr
|
| splitratio |
1/10
|
|
Motoyama, T., Hojo, H. & Watanabe, H. Comparison of seven cell lines derived from human gastric carcinomas. Acta Pathol. Jpn. 36:65-83(1986). Motoya, T., Hojo, H., Suzuki, T. and Oboshi, S. Evaluation of the regrowth assay method as an in vitro drug sensitivity test and its application to cultured human gastric cancer cell lines. Acta Med. Biol., 27, 49-63(1979). Naktani H, Tahara E, Yoshida T et al. Jpn. J. Cancer Res. 77:849-853(1986). Sakurai Y, Hirohasi S, Simosato Y et al. Cancer Res. 48:4053-4058(1988).
|
|---|
