H4-II-E
| KCLB NO |
21548
|
|---|---|
| Name |
H4-II-E
|
세포주 분양조건
서비스
- 서비스 준비중입니다.
수량
-
+
| Origin |
liver
|
|---|---|
| Species |
rat
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
hepatoma
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Eagle's MEM with non-essential amino acids and Earle's BSS, 80%; fetaal bovine serum, 10%, calf serum, 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
