세포주 정보

GH3
GH3
KCLB NO
10082.1
Name
GH3
세포주 분양조건
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Origin
pituitary gland
Species
rat female, 7 months old
Strain
Winstar - Furth
VirusSusceptibility
herpes simplex, vesicular stomatitis(Indiana)
Virus Resistance
poliovirus 1
Reverse Transcriptase
negative
Cellular morphology
epithelial
Production
prolactin,; growth hormone( somatotrophin)
Growth Pattern
monolayer
Histopathology
tumor
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
Ham
kclb media
Dulbecco’s Modified Eagle Medium, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing
Remove culture medium and rinse the cell sheet with fresh 0.25 % trypsin. Remove trypsin, and allow the culture to sit at 37
Bancroft FC et al. Control of growth hormone product ion by a clonal strain of rat pituitary cells. Stimulation by hydrocortisone.J. Cell Biol. 43: 432-441, 1969 PubMed: 70033769RF 10796: Tashjian AH J et al. Production of both prolactin and growth hormone by clonal strains of rat pituitary tumors cells. J. Cell Biol. 47: 61-70, 1970.