Sp2/0-Ag14
| KCLB NO |
21581
|
|---|---|
| Name |
Sp2/0-Ag14
|
세포주 분양조건
서비스
수량
-
+
| Origin |
B cell; B lymphocyte
|
|---|---|
| Species |
mouse
|
| Strain |
BALB/c (B cell); BALB/c (myeloma)
|
| Cellular morphology |
lymphoblast
|
| Histocompatibility |
H-2d
|
| Growth Pattern |
suspension
|
| Histopathology |
hybridoma; mouse(myeloma)
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco's modified Eagle's Medium, with 4.5g/L glucose, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
|
| note |
the line was formed by fusing BALB/c spleen cells (from mouse immunized with sheep RBCs) with the P3X63Ag8 myeloma; the cells do not secrete immunoglobulin, are resistant to 8-azaguanine at 20 mcg/ml and are HAT sensitive; Sp2/0-Ag14 cells can be used as fusion partners for B cells in the production of hybridomas; tested and found negative for ectromelia virus (mousepox)
|
|
J Immunol, 126:317-321, 1981
|
|---|
