NCI-H460
| KCLB NO |
30177
|
|---|---|
| Name |
NCI-H460
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lung
|
|---|---|
| Species |
human male
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolaer
|
| Histopathology |
large cell carcinoma
|
| D3S1358 |
15,18
|
|---|---|
| vWA |
17
|
| FGA |
21,23
|
| Amelogenin |
X,Y
|
| TH01 |
9.3
|
| TPOX |
8
|
| CSF1P0 |
11,12
|
| D5S818 |
9,10
|
| D13S317 |
13
|
| D7S820 |
9,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
|
| splitratio |
1:4
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density)
|
