SNU-685
| KCLB NO |
00685
|
|---|---|
| Name |
SNU-685
|
세포주 분양조건
서비스
수량
-
+
| Origin |
endometrium
|
|---|---|
| Species |
human, asian female, 70 years old, Mongoloid
|
| Cellular morphology |
polypoid
|
| Growth Pattern |
monolayer
|
| Histopathology |
malignant mixed mullerian tumor (MMMT), primary
|
| Growth rate |
fast
|
| Depositor |
Jae-Gahb Park, M.D.
|
| D3S1358 |
14
|
|---|---|
| FGA |
21
|
| Amelogenin |
X
|
| TH01 |
7
|
| TPOX |
9
|
| CSF1P0 |
11
|
| D5S818 |
12
|
| D13S317 |
8
|
| D7S820 |
8,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
| note |
Cytokeratin and EMA were negative, and vimentin and desmin were positive by immunohistochemistry.
|
| populaton doubling time |
20 hours
|
| splitratio |
1:4
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Yuan et al., Establishment and characterization of cell lines derived from uterine malignant mixed mullerian tumor, Gynecol Oncol. 1997 Sep; 66(3): 464-474
|
|
|---|---|
|
