세포주 정보

SNU-NCC-19 (NCC-19)
SNU-NCC-19 (NCC-19)
KCLB NO
50019
Name
SNU-NCC-19 (NCC-19)
세포주 분양조건
서비스
수량
- +
Origin
stomach, gastric
Species
human, asian male, 33 years old
Growth Pattern
monolayer
Depositor
Jae-Gahb Park, M.D.
Option
culture only
D3S1358
16
vWA
18
FGA
20,23
Amelogenin
X,Y
TH01
6,9
TPOX
9
CSF1P0
10,12
D5S818
11
D13S317
8,13
D7S820
8,12
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
media change
remove two thirds of medium, add fresh medium (depending on cell density) every 2 to 3 days
Cell Oncol (Dordr). 2012 Apr;35(2):127-36.