세포주 정보

14

15,16

20,21

X,Y

7,9

9,11

8.12

12,13

8,11

10,11

RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%

RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%

RPMI1640 90%, FBS 10%; Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution ( 1 to 2 ml), and let the flask sit at room temperature ( or incubate at 37

This line is a gastric carcinoma cell line derived in 1976 and associates at the National Cancer Institute from a liver metastasis of a well differentiated carcinoma of the stomach taken prior to cytotoxic therapy. The tumor was passaged as a xenograft in athymic nude mice for three passages before the cell line was established. The cells are L-dopa decarboxylase(DDC) negative. This lines were minimally positive for vasoactive intestinal peptide(VIP) receptors and lacked gastrin receptors. They expressed receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted with the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of C-myc and c-erb-B2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. This lines have a reported plating efficiency of 4.3%. They grow as an adherent monolayer of tightly knit epithelial cells.

47 hrs

1:3 to 1:4

every 2 to 3 days