HT-29
| KCLB NO |
30038
|
|---|---|
| Name |
HT-29
|
세포주 분양조건
서비스
수량
-
+
| Origin |
colon
|
|---|---|
| Species |
human female, 44 years old, Caucasian
|
| Receptor |
urokinase receptor (u-PAR); vitamin D (moderate expression)
|
| VirusSusceptibility |
human immunodeficiency virus (HIV,LAV)
|
| Tumorigenecity |
Yes, in nude mice forms well differentiated adenocarcinoma consistent with colonic primary(grade I); tumors also form in steroid treated hamsters.
|
| Isoenzyme |
Me-2,1;PGM3,1-2;PGM1,1-2;ES-D,1;AK-1,1;GLO-1,1-2;G6PD,B
|
| Cellular morphology |
epithelial
|
| Production |
Secretory component of IgA; carcinoembryonic antigen(CEA); transforming growth factor beta binding protein; mucin
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma
|
| Oncogene |
myc +; ras +; myb +; fos +; sis +;p 53 +; abl -;ros -;src -
|
| D3S1358 |
15,17
|
|---|---|
| vWA |
17,19
|
| FGA |
20,22
|
| Amelogenin |
X
|
| TH01 |
6,9
|
| TPOX |
8,9
|
| CSF1P0 |
11,12
|
| D5S818 |
11,12
|
| D13S317 |
11,12
|
| D7S820 |
10
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
McCoy's 5a medium with 1.5mM L-glutamine, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
|
| splitratio |
1:3 to 1:8 is recommended
|
| media change |
2 to 3 times weekly
|
|
Fogh J., ed., Human tumor cells in vitro. New York: Plenum Press; 1975: pp.115-159Chen TR et al. WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet.Cytogenet. 27: 125-134, 1987
|
|---|
