SW480E
| KCLB NO |
60067
|
|---|---|
| Name |
SW480E
|
세포주 분양조건
서비스
수량
-
+
| Origin |
colon
|
|---|---|
| Species |
human
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
colorectal carcinoma
|
| Depositor |
Prof. Untae Kim
|
| EstablishedBy |
Prof. Untae Kim
|
| D3S1358 |
15
|
|---|---|
| vWA |
16
|
| FGA |
24
|
| Amelogenin |
X
|
| TH01 |
8
|
| TPOX |
11
|
| CSF1P0 |
13,14
|
| D5S818 |
13
|
| D13S317 |
12
|
| D7S820 |
8
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37'C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
|
For the more information for this cell line, please see the reference (metastasis-cell lines, Untaekim.pdf) uploaded in the KCLB web.
|
|---|
