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RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%

RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%

RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%

Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove. Let the culture sit at room temperature or 37

this line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma; the cells were isolated in a chemically defined medium(HITES) and later adapted to growth in media supplemented with serum; the cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation; the cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylase; the line has been selected as a prototype for transfecting human subgenomic fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer; the cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein

48 hrs

1:3 to 1:8

every 5 to 6 days