IEC-18
| KCLB NO |
21589
|
|---|---|
| Name |
IEC-18
|
세포주 분양조건
서비스
- 서비스 준비중입니다.
수량
-
+
| Origin |
ileum; epithelial
|
|---|---|
| Species |
rat
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
normal
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco
|
| kclb media |
DMEM 90%; fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, rinse monolayer with fresh 0.25 % trypsin, 0.02 M EDTA solution, remove trypsin, add fresh medium, aspirate and dispense into new flasks.
|
|
Quaroni A et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J Cell Biol. 80: 248-265, 1979. Quaroni A and Isselbacher HJ. Cytotoxic effects and metabolism of benzopyrene and 7,12 - dimethylbenzanthracene in duodenal and ileal epithelial cell cultures. J. Natl. Cancer Inst. 67: 1353-1362, 1981.
|
|---|
