NCI-H78; HuT 78
| KCLB NO |
90078
|
|---|---|
| Name |
NCI-H78; HuT 78
|
세포주 분양조건
서비스
수량
-
+
| Origin |
T cell; T lymphocyte
|
|---|---|
| Species |
human male, 53 years old, Caucasian
|
| Receptor |
interleukin-2 (IL-2)
|
| Cellular morphology |
lymphoblastoid
|
| Production |
interleukin-2 (IL-2); tumor necrosis factor alpha (TNF-alpha)
|
| Growth Pattern |
suspension
|
| Histopathology |
lymphoma; cutaneous
|
| Requirement |
2mM L-glutamine adjusted to contain 1.5g/L sodium bicarbonate, 4.5g/L glucose, 10mM HEPES, 1.0mM sodium pyruvate
|
| Depositor |
Adi F Gazdar
|
| DateOfUpdated |
1999-02-05
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| kclb media |
Dulbecco’s Modified Eagle Medium, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10^5 cells/ml and maintain between 1 x 10^5 and 1 x 10^6 cells/ml.
|
| note |
Derived from peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2 and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut 78.
|
| media change |
every 2 to 3 days
|
|
Gootenberg JE el at. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. Gazdar AF et al. Mitogen requirements for the in vitro propagation of cutaenous T-cell lymphomas. Blood 55: 409-417, 1980. Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Isnt. 84: 1922-1926, 1992. O
|
|---|
