Hs 294T
| KCLB NO |
30140
|
|---|---|
| Name |
Hs 294T
|
세포주 분양조건
서비스
수량
-
+
| Origin |
skin
|
|---|---|
| Species |
human male, 56 years old, caucasian
|
| Receptor |
nerve growth factor; interferon
|
| Tumorigenecity |
yes, in immunosuppressed NAMRU mice
|
| Isoenzyme |
G6PD, B; PGM1, 1-2; PGM3, 1; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 1-2
|
| Karyology |
(P26) heteroploid with modal number=59; Y chromosomes present
|
| Cellular morphology |
mixed stellate and polygonal
|
| Growth Pattern |
monolayer
|
| Histopathology |
melanoma; metastatic to lymph node
|
| D3S1358 |
16,17
|
|---|---|
| vWA |
16,18
|
| FGA |
23,24
|
| Amelogenin |
X
|
| TH01 |
8,9.3
|
| TPOX |
8,11
|
| CSF1P0 |
11
|
| D5S818 |
12
|
| D13S317 |
11,12
|
| D7S820 |
10
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco
|
| kclb media |
DMEM 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| note |
the proliferative of the cell ine is enhanced by addition of retinoic acid; responds to mitogenic stimulation by phytohemagglutinin and forms colonies in soft agar medium or on contact inhibited monolayers. The melanoma cells have also been shown to be responsive to the anti proliferative activity of interferon; results from a competitive binding study indicated that Hs 294T cells possess at least two types of interferon (IFN) receptors, one which binds IFN alpha and IFN beta and another which binds IFN gamma
Penicillin-streptomycin were added to culture media (final concentration, 100 U/mL)
|
|
In Vitro, 15:342-350, 1979; In Vitro, 15;565-568, 1979
|
|---|
