소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-354

KCLB No. 00354
Name SNU-354
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin liver
Species human, asian - male, 52 years old
Abnormal genes HBX gene
Virus Hepatitis B virus (HBV)
Cellular morphology polygonal
Growth pattern adherent
Histopathology carcinoma, hepatocellular; primary
Differentiation grade 2-3/4
Requirement humidified incubators at 37
Modal Choromosome No 78
Depositor Jae-Gahb Park, M.D.
Option culture only
D3S1358: 15,17
vWA: 17
FGA: 21,22
Amelogenin: X,Y
TH01: 9
TPOX: 11
CSF1PO: 8,11
D5S818: 11,12
D13S317: 10,11
D7S820: 7,11
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Populaton doubling time 34 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Park et al., Characterization of cell lines established from human hepatocellular carcinoma. Int J Cancer, 62: 276-282, 1995; Kang et al., Mutation of p53 gene in hepatocellular carcinoma cell lines with HBX DNA. Int. J. Cancer, 67: 898-902, 1996
  • Park JG, Lee JH, Kang MS, Park KJ, Jeon YM, Lee HJ, Kwon HS, Park HS, Yeo KS, Lee KU, et al: Characterization of cell lines established from human hepatocellular carcinoma. Int J Cancer 28: 62(3), 276-82. Jul 1995
    ▶ Ref. KCLB Cell line No.'s - [ 00354, 00368 ]

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