소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-475

KCLB No. 00475
Name SNU-475
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin liver
Species human, asian - male, 43 years old
Virus Hepatitis B virus (HBV)
Cellular morphology epithelial
Growth pattern monolayer
Histopathology carcinoma, hepatocellular; primary
Depositor Jae-Gahb Park, M.D.
D3S1358: 14
vWA: 14
FGA: 23
Amelogenin: X,Y
TH01: 7,9
TPOX: 8,9
CSF1PO: 11,12
D5S818: 10,13
D13S317: 8,11
D7S820: 7,12
no image
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
Note Hepatitis B virus integrated.
Populaton doubling time 66 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Park et al., Characterization of cell lines established from human hepatocellular carcinoma. Int J Cancer, 62: 276-282, 1995; Kang et al., Mutation of p53 gene in hepatocellular carcinoma cell lines with HBX DNA. Int. J. Cancer, 67: 898-902, 1996

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