소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-685

KCLB No. 00685
Name SNU-685
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin endometrium
Species human, asian - female, 70 years old, Mongoloid
Cellular morphology polypoid
Growth pattern monolayer
Histopathology malignant mixed mullerian tumor (MMMT), primary
Growth rate fast
Depositor Jae-Gahb Park, M.D.
D3S1358: 14
FGA: 21
Amelogenin: X
TH01: 7
TPOX: 9
CSF1PO: 11
D5S818: 12
D13S317: 8
D7S820: 8,12
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
Note Cytokeratin and EMA were negative, and vimentin and desmin were positive by immunohistochemistry.
Populaton doubling time 20 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Yuan et al., Establishment and characterization of cell lines derived from uterine malignant mixed mullerian tumor, Gynecol Oncol. 1997 Sep; 66(3): 464-474
  • Yuan Y, Kim WH, Han HS, Lee JH, Park HS, Chung JK, Kang SB, Park JG: Establishment and characterization of cell lines derived from uterine malignant mixed Mullerian tumor. Gynecol Oncol 66: 3, 464-74. Sep 1997
    ▶ Ref. KCLB Cell line No.'s - [ 00539, 00685, 01077 ]

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